De novo biosynthesis of glycosaminoglycans in the extracellular matrix of skin studied by matrix-assisted laser desorption/ionization mass spectrometry

Anal Biochem. 2012 Feb 15;421(2):791-3. doi: 10.1016/j.ab.2011.11.002. Epub 2011 Nov 12.


The self-healing capacity of skin is limited, and medical intervention is often unavoidable. Skin may be generated ex vivo from cultured fibroblasts. Because the molecular composition of de novo formed skin (mostly collagen and glycosaminoglycans [GAGs]) is crucial, analytical methods are required for the quality control of tissue-engineered products. Here, we show that matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) of fibroblast cultures subsequent to digestion with chondroitinase ABC is a reliable and fast method to monitor the GAG content of native and bioengineered skin. Furthermore, the supplementation of the fibroblast medium with ¹³C-labeled glucose provides insights into the biosynthesis of GAGs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Chondroitin ABC Lyase / analysis*
  • Culture Media
  • Extracellular Matrix / metabolism*
  • Fibroblasts / enzymology*
  • Glucose / chemistry
  • Glycosaminoglycans / biosynthesis*
  • Humans
  • Skin / metabolism*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*


  • Culture Media
  • Glycosaminoglycans
  • Chondroitin ABC Lyase
  • Glucose