Controlled Gene Expression in Primary Lgr5 Organoid Cultures

Nat Methods. 2011 Dec 4;9(1):81-3. doi: 10.1038/nmeth.1802.

Abstract

The study of gene function in endodermal epithelia such as of stomach, small intestine and colon relies heavily on transgenic approaches. Establishing such animal models is laborious, expensive and time-consuming. We present here a method based on Cre recombinase-inducible retrovirus vectors that allows the conditional manipulation of gene expression in primary mouse organoid culture systems.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Gene Expression
  • Gene Knockdown Techniques / methods
  • Green Fluorescent Proteins / genetics
  • Humans
  • Integrases / metabolism
  • Intestinal Mucosa / cytology*
  • Mice
  • Organoids / cytology*
  • Organoids / metabolism
  • Receptors, G-Protein-Coupled / physiology*
  • Receptors, Notch / physiology
  • Retroviridae / genetics
  • Stem Cells / virology
  • Tamoxifen / analogs & derivatives
  • Tamoxifen / pharmacology
  • Transduction, Genetic / methods

Substances

  • Lgr5 protein, mouse
  • Receptors, G-Protein-Coupled
  • Receptors, Notch
  • enhanced green fluorescent protein
  • Tamoxifen
  • Green Fluorescent Proteins
  • afimoxifene
  • Cre recombinase
  • Integrases