Interpretation Guidelines of a Standard Y-chromosome STR 17-plex PCR-CE Assay for Crime Casework

Methods Mol Biol. 2012;830:43-56. doi: 10.1007/978-1-61779-461-2_4.

Abstract

Y-STR analysis is an invaluable tool to examine evidence in sexual assault cases and in other forensic casework. Unambiguous detection of the male component in DNA mixtures with a high female background is still the main field of application of forensic Y-STR haplotyping. In the last years, powerful technologies including a 17-locus multiplex PCR assay have been introduced in the forensic laboratories. At the same time, statistical methods have been developed and adapted for interpretation of a nonrecombining, linear marker as the Y-chromosome which shows a strongly clustered geographical distribution due to the linear inheritance and the patrilocality of ancestral groups. Large population databases, namely the Y-STR Haplotype Reference Database (YHRD), have been established to assess the evidentiary value of Y-STR matches by means of frequency estimation methods (counting and extrapolation).

MeSH terms

  • Chromosomes, Human, Y / genetics*
  • Confidence Intervals
  • Crime*
  • DNA / genetics
  • Electrophoresis, Capillary / methods*
  • Female
  • Forensic Genetics / methods*
  • Genetic Loci / genetics
  • Geography
  • Guidelines as Topic*
  • Haplotypes / genetics
  • Humans
  • Likelihood Functions
  • Male
  • Microsatellite Repeats / genetics*
  • Polymerase Chain Reaction / methods*
  • Reference Standards

Substances

  • DNA