Bacteriocin production and resistance to drugs are advantageous features for Lactobacillus acidophilus La-14, a potential probiotic strain

New Microbiol. 2011 Oct;34(4):357-70. Epub 2011 Oct 31.


L. acidophilus La-14 produces bacteriocin active against L. monocytogenes ScottA (1600 AU/ml) in MRS broth at 30°C or 37°C. The bacteriocin proved inhibitory to different serological types of Listeria spp. Antimicrobial activity was completely lost after treatment of the cell-free supernatant with proteolytic enzymes. Addition of bacteriocin produced by L. acidophilus La-14 to a 3 h-old culture of L. monocytogenes ScottA repressed cell growth in the following 8h. Treatment of stationary phase cells of L. monocytogenes ScottA (107-108 CFU/ml) by the bacteriocin resulted in growth inhibition. Growth of L. acidophilus La-14 was not inhibited by commercial drugs from different generic groups, including nonsteroidal anti-inflammatory drugs (NSAID) containing diclofenac potassium or ibuprofen arginine. Only one non-antibiotic drug tested, Atlansil (an antiarrhythmic agent), had an inhibitory effect on L. acidophilus La-14 with MIC of 2.5 mg/ml. L. acidophilus La-14 was not affected by drugs containing sodium or potassium diclofenac. L. acidophilus La-14 shows a good resistance to several drugs and may be applied in combination for therapeutic use.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analgesics / pharmacology
  • Anti-Arrhythmia Agents / pharmacology
  • Anti-Inflammatory Agents, Non-Steroidal / pharmacology
  • Bacteriocins / biosynthesis*
  • Bacteriocins / pharmacology
  • Detergents / pharmacology
  • Drug Resistance, Bacterial / physiology*
  • Enzymes / pharmacology
  • Hydrogen-Ion Concentration
  • Lactobacillus acidophilus* / drug effects
  • Lactobacillus acidophilus* / growth & development
  • Lactobacillus acidophilus* / metabolism
  • Listeria monocytogenes* / drug effects
  • Listeria monocytogenes* / growth & development
  • Listeria monocytogenes* / metabolism
  • Microbiological Techniques
  • Probiotics / metabolism*
  • Probiotics / therapeutic use
  • Quorum Sensing / drug effects
  • Temperature


  • Analgesics
  • Anti-Arrhythmia Agents
  • Anti-Inflammatory Agents, Non-Steroidal
  • Bacteriocins
  • Detergents
  • Enzymes