Isolation of undifferentiated and early differentiating type A spermatogonia from Pou5f1-GFP reporter mice

Methods Mol Biol. 2012;825:31-44. doi: 10.1007/978-1-61779-436-0_3.


Limited understanding of the mechanisms underlying self-renewal and differentiation of spermatogonial stem cells hampers our ability to develop new therapeutic and contraceptive approaches. Mouse models of spermatogonial stem cell development are key to developing new insights into the biology of both the normal and diseased testis. Advances in transgenic reporter mice have enabled the isolation, molecular characterization, and functional analysis of mouse Type A spermatogonia subpopulations from the normal testis, including populations enriched for spermatogonial stem cells. Application of these reporters both to the normal testis and to gene-deficient and over-expression models will promote a better understanding of the earliest steps of spermatogenesis, and the role of spermatogonial stem cells in germ cell tumor.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cell Differentiation*
  • Cell Separation / methods*
  • Flow Cytometry
  • Genes, Reporter*
  • Green Fluorescent Proteins / genetics*
  • Male
  • Mice
  • Mice, Transgenic
  • Octamer Transcription Factor-3 / genetics*
  • Octamer Transcription Factor-3 / metabolism
  • Proto-Oncogene Proteins c-kit / metabolism
  • Spermatogenesis / genetics
  • Spermatogonia / cytology*
  • Spermatogonia / metabolism*


  • Octamer Transcription Factor-3
  • Pou5f1 protein, mouse
  • Green Fluorescent Proteins
  • Proto-Oncogene Proteins c-kit