Hepatoprotective Potential of Clitoria Ternatea Leaf Extract Against Paracetamol Induced Damage in Mice

Molecules. 2011 Dec 6;16(12):10134-45. doi: 10.3390/molecules161210134.

Abstract

Background and aim: Clitoria ternatea, a medicinal herb native to tropical equatorial Asia, is commonly used in folk medicine to treat various diseases. The aim of the present study is to evaluate the hepatoprotective and antioxidant activity of C. ternatea against experimentally induced liver injury.

Methods: The antioxidant property of methanolic extract (ME) of C. ternatea leaf was investigated by employing an established in vitro antioxidant assay. The hepatoprotective effect against paracetamol-induced liver toxicity in mice of ME of C. ternatea leaf was also studied. Activity was measured by monitoring the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and billirubin along with histopathological analysis.

Results: The amount of total phenolics and flavonoids were estimated to be 358.99 ± 6.21 mg/g gallic acid equivalent and 123.75 ± 2.84 mg/g catechin equivalent, respectively. The antioxidant activity of C. ternatea leaf extract was 67.85% at a concentration of 1 mg/mL and was also concentration dependant, with an IC(50) value of 420.00 µg/mL. The results of the paracetamol-induced liver toxicity experiments showed that mice treated with the ME of C. ternatea leaf (200 mg/kg) showed a significant decrease in ALT, AST, and bilirubin levels, which were all elevated in the paracetamol group (p < 0.01). C. ternatea leaf extract therapy also protective effects against histopathological alterations. Histological studies supported the biochemical findings and a maximum improvement in the histoarchitecture was seen.

Conclusions: The current study confirmed the hepatoprotective effect of C. ternatea leaf extract against the model hepatotoxicant paracetamol. The hepatoprotective action is likely related to its potent antioxidative activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetaminophen
  • Animals
  • Antioxidants / metabolism
  • Bilirubin / blood
  • Biomarkers / metabolism
  • Biphenyl Compounds / metabolism
  • Butylated Hydroxytoluene / metabolism
  • Chemical and Drug Induced Liver Injury / blood
  • Chemical and Drug Induced Liver Injury / drug therapy*
  • Chemical and Drug Induced Liver Injury / pathology
  • Clitoria / chemistry*
  • Flavonoids / analysis
  • Free Radical Scavengers / metabolism
  • Inhibitory Concentration 50
  • Liver / drug effects
  • Liver / enzymology
  • Liver / pathology
  • Male
  • Mice
  • Phenols / analysis
  • Phytotherapy
  • Picrates / metabolism
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use*
  • Plant Leaves / chemistry*
  • Protective Agents / pharmacology
  • Protective Agents / therapeutic use*
  • Reference Standards

Substances

  • Antioxidants
  • Biomarkers
  • Biphenyl Compounds
  • Flavonoids
  • Free Radical Scavengers
  • Phenols
  • Picrates
  • Plant Extracts
  • Protective Agents
  • Butylated Hydroxytoluene
  • Acetaminophen
  • 1,1-diphenyl-2-picrylhydrazyl
  • Bilirubin