Down-regulation of heme oxygenase-1 by SVCV infection

Fish Shellfish Immunol. 2012 Feb;32(2):301-6. doi: 10.1016/j.fsi.2011.11.019. Epub 2011 Nov 27.

Abstract

Heme oxygenase (HO-1) is a cytoprotective enzyme that plays a critical role in defending the body against oxidant-induced injury during inflammatory processes. In mammalian systems, viral infection or antigen expression can down-regulate the expression of HO-1. In turn, the induction of HO-1 or overexpression of HO-1 results in potent and direct antiviral activity that targets the replication of several mammalian viruses. In this study, the HO-1 gene of Cyprinus carpio was cloned, and the expression profile of HO-1 was investigated during spring viremia of carp virus (SVCV) infection. The results demonstrate that the expression of HO-1 was down-regulated during SVCV infection in the EPC cells and in common carp. These results indicated that SVCV infection could induce host oxidative stress, which may contribute to tissue injury in affect fish.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Carps / classification
  • Carps / genetics*
  • Carps / metabolism*
  • Cell Line
  • Down-Regulation*
  • Fish Diseases / enzymology*
  • Fish Diseases / immunology
  • Gene Expression Profiling
  • Heme Oxygenase-1 / chemistry
  • Heme Oxygenase-1 / genetics*
  • Heme Oxygenase-1 / metabolism
  • Molecular Sequence Data
  • Phylogeny
  • Rhabdoviridae Infections / enzymology
  • Rhabdoviridae Infections / immunology
  • Rhabdoviridae Infections / veterinary*
  • Sequence Alignment
  • Vesiculovirus / immunology

Substances

  • Heme Oxygenase-1