[Ethyl pyruvate inhibited HMGB1 expression induced by LPS in macrophages]

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2011 Dec;27(12):1304-7, 1311.
[Article in Chinese]


Aim: To elucidate the mechanism of ethyl pyruvate (EP) inhabit high mobility group protein B1 (HMGB1)expression and releasing in macrophage induced by lipopolysaccharide(LPS).

Methods: The murine macrophage-like cell line RAW264.7 cultured in vitro divided into LPS group and LPS+EP group. The expression of HMGB1 mRNA in cultured cell was determined by RT-PCR. The cytoplasmic and nuclear HMGB1 levels were detected by Western blot. The contents of HMGB1 and TNF-α and IL-6 protein in cultured cells supernatant were detected by ELISA. Immunocytochemistry and confocal laser-scanning microscopy were used to confirm the relocation and distribution of intracellular HMGB1 protein in RAW264.7 cells.

Results: HMGB1 mRNA expression in the LPS+EP group was significantly lower than in LPS alone, at 24, 36 and 48 hours. In the LPS+EP stimulation group, the cytoplasm stained weakly while the nuclear stain was stronger than that of the LPS group at the same time points. Both TNF-α and IL-6 levels in LPS+EP group were significantly lower than those in the LPS group at the same time points. EP also effectively prevented the release of HMGB1 protein.

Conclusion: EP inhibits HMGB1 expression and release from LPS-stimulated macrophages.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • HMGB1 Protein / antagonists & inhibitors*
  • HMGB1 Protein / biosynthesis
  • Interleukin-6 / biosynthesis
  • Lipopolysaccharides / pharmacology*
  • Macrophages / drug effects*
  • Macrophages / metabolism
  • Mice
  • Pyruvates / pharmacology*
  • Tumor Necrosis Factor-alpha / biosynthesis


  • HMGB1 Protein
  • Interleukin-6
  • Lipopolysaccharides
  • Pyruvates
  • Tumor Necrosis Factor-alpha
  • ethyl pyruvate