Anti-inflammatory and antioxidative effects of mucilage of Trigonella foenum graecum (Fenugreek) on adjuvant induced arthritic rats

Int Immunopharmacol. 2012 Jan;12(1):205-11. doi: 10.1016/j.intimp.2011.11.012. Epub 2011 Dec 7.


The aim of this study was to investigate anti-inflammatory and antioxidant effects of mucilage from fenugreek in adjuvant induced arthritis in rats. Arthritis was induced by intradermal injection of complete Freund's adjuvant into the right hind paw produce inflammation of the joint. The activities of inflammatory enzymes like cyclooxygenase, lipoxygenase and myeloperoxidase, and levels of nitrite and C-reactive protein were observed. Also oxidative stress was measured by analyzing the activity of catalase, superoxide dismutase, glutathione peroxidase and the levels of glutathione and vitamin C and lipid peroxidation. The blood parameters like ESR, total WBC, RBC and hemoglobin content was checked. Fenugreek mucilage exhibited maximum percentage of edema inhibition at a dose of 75 mg/kg on 21st day of adjuvant arthritis. The effect was higher than that of standard drug indomethacin. The activities of cyclooxygenase-2 and myeloperoxidase and concentration of thiobarbituric acid reactive substance (TBARS) were decreased and the activities of antioxidant enzymes, vitamins C and reduced glutathione level were increased on treatment with fenugreek mucilage. The increment in ESR and total WBC, reduction in RBC count and hemoglobin and aberrant changes to the C-reactive protein (CRP) levels observed in the arthritic animals were also found to be significantly restored in fenugreek mucilage treated rats. Histopathology of paw tissue showed decreased edema formation and cellular infiltration on supplementation with fenugreek mucilage. Thus the results demonstrated the potential beneficiary effect of fenugreek mucilage on adjuvant induced arthritis in rats.

MeSH terms

  • Alanine Transaminase / metabolism
  • Animals
  • Anti-Inflammatory Agents / therapeutic use*
  • Antioxidants / therapeutic use*
  • Arachidonate 5-Lipoxygenase / metabolism
  • Arthritis, Experimental / drug therapy*
  • Arthritis, Experimental / metabolism
  • Arthritis, Experimental / pathology
  • Aspartate Aminotransferases / metabolism
  • Blood Cell Count
  • C-Reactive Protein / analysis
  • Carrageenan
  • Cyclooxygenase 2 / metabolism
  • Edema / chemically induced
  • Edema / drug therapy
  • Edema / metabolism
  • Edema / pathology
  • Female
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / enzymology
  • Nitrites / blood
  • Oxidative Stress / drug effects
  • Peroxidase / metabolism
  • Phytotherapy*
  • Plant Extracts / therapeutic use*
  • Rats
  • Rats, Sprague-Dawley
  • Seeds
  • Trigonella*


  • Anti-Inflammatory Agents
  • Antioxidants
  • Nitrites
  • Plant Extracts
  • Carrageenan
  • C-Reactive Protein
  • Peroxidase
  • Arachidonate 5-Lipoxygenase
  • Cyclooxygenase 2
  • Ptgs2 protein, rat
  • Aspartate Aminotransferases
  • Alanine Transaminase