A systems-biology approach to yeast actin cables

Adv Exp Med Biol. 2012;736:325-35. doi: 10.1007/978-1-4419-7210-1_19.

Abstract

We focus on actin cables in yeast as a model system for understanding cytoskeletal organization and the workings of actin itself. In particular, we highlight quantitative approaches on the kinetics of actin-cable assembly and methods of measuring their morphology by image analysis. Actin cables described by these studies can span greater lengths than a thousand end-to-end actin-monomers. Because of this difference in length scales, control of the actin-cable system constitutes a junction between short-range interactions - among actin-monomers and nucleating, polymerization-facilitating, side-binding, severing, and cross-linking proteins - and the emergence of cell-scale physical form as embodied by the actin cables themselves.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Actins / chemistry
  • Actins / metabolism
  • Actins / physiology*
  • Cell Cycle Proteins / metabolism
  • Cytoplasm / metabolism
  • Fungal Proteins / chemistry
  • Fungal Proteins / metabolism
  • Fungal Proteins / physiology*
  • Kinetics
  • Models, Biological
  • Protein Multimerization
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae / physiology
  • Schizosaccharomyces / metabolism
  • Schizosaccharomyces / physiology
  • Signal Transduction / physiology*
  • Species Specificity
  • Systems Biology / methods*
  • Yeasts / metabolism
  • Yeasts / physiology*

Substances

  • Actins
  • Cell Cycle Proteins
  • Fungal Proteins