Specific expression of the ret proto-oncogene in human neuroblastoma cell lines

Oncogene. 1990 Sep;5(9):1291-6.


The expression of the ret proto-oncogene (proto-ret), which possibly encodes two isoforms of a receptor-type tyrosine kinase, was examined in human tumor cell lines. Expression of the proto-ret mRNA was detected in all 11 neuroblastoma cell lines examined. The level of mRNA varied more than 100-fold in these neuroblastoma cell lines and was particularly high in three of them. On the other hand, 19 non-neuroblastoma tumor cell lines derived from solid tumors and a human diploid fibroblast cell line did not express any detectable levels of proto-ret mRNA. No remarkable amplification of the proto-ret or gross structural changes in the coding region were found in these neuroblastoma cell lines. The specific expression of the proto-ret in neuroblastomas suggests that the proto-ret product may have a role in cellular functions specific to neuroblastoma cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Blotting, Southern
  • DNA Probes
  • Drosophila Proteins*
  • Gene Amplification*
  • Gene Expression Regulation, Neoplastic*
  • Genes, myc
  • Humans
  • Neuroblastoma / genetics*
  • Phosphopyruvate Hydratase / genetics
  • Protein-Tyrosine Kinases / genetics
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins c-ret
  • RNA, Messenger / analysis*
  • RNA, Neoplasm / analysis*
  • Receptor Protein-Tyrosine Kinases*
  • Tumor Cells, Cultured


  • DNA Probes
  • Drosophila Proteins
  • Proto-Oncogene Proteins
  • RNA, Messenger
  • RNA, Neoplasm
  • Protein-Tyrosine Kinases
  • Proto-Oncogene Proteins c-ret
  • Receptor Protein-Tyrosine Kinases
  • Ret protein, Drosophila
  • Phosphopyruvate Hydratase