Delivery of multiple siRNAs using lipid-coated PLGA nanoparticles for treatment of prostate cancer

Nano Lett. 2012 Jan 11;12(1):287-92. doi: 10.1021/nl2035354. Epub 2011 Dec 21.


Nanotechnology can provide a critical advantage in developing strategies for cancer management and treatment by helping to improve the safety and efficacy of novel therapeutic delivery vehicles. This paper reports the fabrication of poly(lactic acid-co-glycolic acid)/siRNA nanoparticles coated with lipids for use as prostate cancer therapeutics made via a unique soft lithography particle molding process called Particle Replication In Nonwetting Templates (PRINT). The PRINT process enables high encapsulation efficiency of siRNA into neutral and monodisperse PLGA particles (32-46% encapsulation efficiency). Lipid-coated PLGA/siRNA PRINT particles were used to deliver therapeutic siRNA in vitro to knockdown genes relevant to prostate cancer.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Coated Materials, Biocompatible / chemical synthesis*
  • Genetic Therapy / methods*
  • Humans
  • Lactic Acid / chemistry
  • Lipids / chemistry
  • Male
  • Nanocapsules / therapeutic use*
  • Polyglycolic Acid / chemistry
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Prostatic Neoplasms / genetics*
  • Prostatic Neoplasms / therapy*
  • RNA, Small Interfering / genetics*
  • RNA, Small Interfering / therapeutic use*


  • Coated Materials, Biocompatible
  • Lipids
  • Nanocapsules
  • RNA, Small Interfering
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Lactic Acid