Rat DNA polymerase beta gene can join in excision repair of Escherichia coli

Nucleic Acids Res. 1990 Oct 11;18(19):5673-6. doi: 10.1093/nar/18.19.5673.


Though DNA polymerase I (poll) of Escherichia (E.) coli is understood to play a role in repair synthesis of excision repair, it is still obscure whether DNA polymerase beta (pol beta) plays a similar role in eukaryotic cells. To estimate the role of pol beta in excision repair processes, we inserted the rat pol beta gene into several mutant E. coli defective in a diverse set of enzymatic activities of poll. UV resistance was seen only when the 5'----3' exonuclease (exo) activity of poll molecules remained. Therefore it is suggested that 5'----3' exo activity as well as pol beta activity are essential for repair synthesis of excision repair in eukaryotic cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA Damage
  • DNA Polymerase I / genetics*
  • DNA Polymerase I / metabolism
  • DNA Repair*
  • DNA, Bacterial / genetics*
  • DNA, Bacterial / metabolism
  • DNA, Bacterial / radiation effects
  • Escherichia coli / genetics*
  • Genes
  • Mutation
  • Plasmids
  • Rats
  • Restriction Mapping
  • Transfection
  • Transformation, Bacterial
  • Ultraviolet Rays


  • DNA, Bacterial
  • DNA Polymerase I