Assignment of backbone resonances in a eukaryotic protein kinase - ERK2 as a representative example

Methods Mol Biol. 2012;831:359-68. doi: 10.1007/978-1-61779-480-3_19.

Abstract

A first step toward the analysis of the structure, dynamics, and interactions of proteins by NMR is obtaining an acceptable level of resonance assignments. This process is nontrivial in most eukaryotic kinases given their size and suboptimal behavior in solution. Using inactive ERK2 as a representative example, we describe the procedures we utilized to achieve a significant degree of completeness of backbone resonance assignment.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Mitogen-Activated Protein Kinase 1 / chemistry*
  • Models, Molecular*
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Protein Conformation*
  • Spin Labels

Substances

  • Spin Labels
  • Mitogen-Activated Protein Kinase 1