Immunostimulatory and protective effects of Aloe vera against coccidiosis in industrial broiler chickens

Vet Parasitol. 2012 May 25;186(3-4):170-7. doi: 10.1016/j.vetpar.2011.11.059. Epub 2011 Nov 25.


This paper reports the immunostimulatory and protective effects of Aloe vera extracts (aqueous and ethanolic) against coccidiosis in industrial broiler chickens. The study was divided into two experiments. Experiment-I was conducted for the evaluation of immunostimulatory activity of A. vera and experiment-II demonstrated the protective efficacy of A. vera extracts against coccidiosis in chickens. Results of the experiment-I revealed significantly higher (p<0.05) lymphoproliferative responses in chickens administered with ethanolic extract of A. vera as compared to those administered with aqueous extract and control group. Microplate haemagglutination assay for humoral response on day 7th and 14th post primary and secondary injections of sheep red blood cells (SRBCs) revealed significantly higher (p<0.05) anti SRBC antibody (total Igs, IgG and IgM) titers in chickens of experimental groups as compared to the control group. None of the extracts, however, demonstrated significant effects on the development of lymphoid organs. Results of experiment-II revealed maximum protection (60%) in chickens administered with aqueous Aloe extract as compared to the ethanolic extract administered chickens (45%). Mean oocysts per gram of droppings in the control group was significantly higher (p<0.05) as compared to the chickens in both the experimental groups. Chickens administered with aqueous Aloe extract showed a minimal mean lesion score (2.3) followed by those administered with ethanolic Aloe extract (2.6) and control chickens (3.05) for caeca, and a similar pattern was observed for intestinal lesion scoring. Further, significantly higher weight gains and antibody titers (p<0.05) were observed in chickens administered with A. vera extracts as compared to those in the control group. It was concluded that A. vera may be a potential and valuable candidate to stimulate the immune responses and can be used successfully as an immunotherapeutic agent against coccidiosis in industrial broiler chickens.

MeSH terms

  • Aloe / chemistry*
  • Animals
  • Body Weight
  • Chickens / immunology
  • Chickens / parasitology*
  • Coccidiosis / immunology
  • Coccidiosis / prevention & control
  • Coccidiosis / veterinary*
  • Coccidiostats / chemistry
  • Coccidiostats / therapeutic use*
  • Lymphoid Tissue / anatomy & histology
  • Lymphoid Tissue / drug effects
  • Organ Size
  • Plant Extracts / chemistry
  • Plant Extracts / therapeutic use*
  • Poultry Diseases / immunology
  • Poultry Diseases / prevention & control*


  • Coccidiostats
  • Plant Extracts