Quantitative evaluation of C-arm CT cerebral blood volume in a canine model of ischemic stroke

AJNR Am J Neuroradiol. 2012 Feb;33(2):353-8. doi: 10.3174/ajnr.A2944. Epub 2011 Dec 15.


Background and purpose: Previous studies have shown the feasibility of assessing qualitative CBV measurements in the angiography suite by using FPD-CBCT systems. We have investigated the correlation of FPD-CBCT CBV lesion volumes to the infarct volume.

Materials and methods: Unilateral strokes were created in 7 adult dogs. MR imaging and FPD-CBCT data were obtained after MCA occlusion. FPD-CBCT CBV and ADC maps were generated for all subjects. The animals were sacrificed immediately following the last imaging study to measure infarct volume on histology. The reliability of FPD-CBCT-based lesion volume measurements was compared with those measured histologically by using regression and Bland-Altman analysis.

Results: The best correlation (R(2) = 0.72) between lesion volumes assessed with FPD-CBCT and histology was established with a threshold of mean healthy CBV - 2.5 × SD. These results were inferior to the correlation of lesion volumes measured with ADC and histology (R(2) = 0.99). Bland-Altman analysis showed that the agreement of ADC-derived lesion volumes with histology was superior to the agreement of FPD-CBCT-derived lesion volumes with histology.

Conclusions: We correlated FPD-CBCT measurements of CBV and MR ADC lesion volumes with histologically assessed infarct volume. As expected, ADC is a very accurate and precise method for determining the extent of infarction. FPD-CBCT CBV lesion volumes are correlated to the size of the infarct. Improvement of FPD-CBCT image quality provides an opportunity to establish quantitative CBV measurement in the angiography suite.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Blood Volume*
  • Brain Ischemia / diagnostic imaging*
  • Brain Ischemia / physiopathology*
  • Disease Models, Animal
  • Dogs
  • Stroke / diagnostic imaging*
  • Stroke / physiopathology*
  • Tomography, X-Ray Computed*