[Effect of Transforming Growth Factor β(1) and Insulin-Like Growth factor-I on Extracelluar Matrix Synthesis of Self-Assembled Constructs of Goat Temporomandibular Joint Disc]

Zhonghua Kou Qiang Yi Xue Za Zhi. 2011 Sep;46(9):541-6. doi: 10.3760/cma.j.issn.1002-0098.2011.09.008.
[Article in Chinese]


Objective: To examine the effects of high and low concentrations of transforming growth factor (TGF) β(1) and insulin-like growth factor-I (IGF-I) on the extracelluar matrix synthesis of the self-assembled constructs of temporomandibular joint (TMJ) disc.

Methods: The experimental groups of self-assembled constructs were exposed to IGF-I (10, 100 µg/L) and TGF-β(1) (5, 50 µg/L), the control groups were not added with any growth factors. All groups were examined at 3 and 6 weeks for gross morphological, histological, and biochemical changes. Safranin-O/fast green staining was used to examine glycosaminoglycan (GAG) distribution, picrosirius red and immunohistochemical staining to observe type I collagen distribution. Type I collagen contents were tested by ELISA assay kit, GAG contents were measured by Blyscan GAG assay kit, and the cell numbers were quantified with a Picogreen reagent kit.

Results: The growth factor groups all upregulated the matrix synthesis of the self-assembled constructs compared with control groups. TGF-β(1) (5 µg/L) and IGF-I (10 µg/L) were the two most potent concentration in increasing type I collagen and GAG synthesis and cells proliferation. IGF-I group (10 µg/L) produced nearly 2 times (109.16 ± 5.12 µg) as much type I collagen as the control group (69.13 ± 5.94 µg) at 3 weeks. The matrix contents and the number of the proliferated cells in control group and all GF groups at 6 weeks were more than those at 3 weeks.

Conclusions: IGF-I (10 µg/L) is the most beneficial growth factor and can be applied in tissue-engineering stratigies of the temporomandibular joint disc. At the same time, the exposure time of growth factors is another key factor that affects matrix synthesis of TMJ disc constructs.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Collagen Type I / biosynthesis*
  • Extracellular Matrix / metabolism*
  • Glycosaminoglycans / biosynthesis*
  • Goats
  • Insulin-Like Growth Factor I / pharmacology*
  • Temporomandibular Joint Disc* / cytology
  • Temporomandibular Joint Disc* / metabolism
  • Tissue Engineering / methods
  • Transforming Growth Factor beta1 / pharmacology*


  • Collagen Type I
  • Glycosaminoglycans
  • Transforming Growth Factor beta1
  • Insulin-Like Growth Factor I