Determination of ABO Genotypes by Real-Time PCR Using Allele-Specific Primers

Leg Med (Tokyo). 2012 Jan;14(1):47-50. doi: 10.1016/j.legalmed.2011.10.002. Epub 2011 Dec 16.


ABO grouping of biological specimens is informative for identifying victims and narrowing down suspects. In Japan and elsewhere, ABO grouping as well as DNA profiling plays an essential role in crime investigations. In the present study, we developed a new method for ABO genotyping using allele-specific primers and real-time PCR. The method allows for the detection of three single nucleotide polymorphisms (SNPs) at nucleotide positions 261, 796, and 803 in the ABO gene and the determination of six major ABO genotypes. This method required less than 2 h for accurate ABO genotyping using 2.0 ng of DNA. This method could be applicable for rapid and simple screening of forensic samples.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ABO Blood-Group System / genetics*
  • Alleles
  • DNA Primers
  • Forensic Genetics / methods
  • Genotype*
  • Humans
  • Polymorphism, Single Nucleotide
  • Real-Time Polymerase Chain Reaction


  • ABO Blood-Group System
  • DNA Primers