Ligand-induced clustering of EGF receptors: a quantitative study by fluorescence image moment analysis

Biophys Chem. 2012 Feb;161:50-3. doi: 10.1016/j.bpc.2011.11.003. Epub 2011 Nov 25.


Fluorescence microscopy is widely used in the life sciences, but largely for qualitative imaging. Here we apply a bioanalytical technique, fluorescence image moment analysis, to demonstrate how the distribution of the fluorescent molecules can be measured directly from confocal microscopy images. We measured the oligomerization state of EGF-eGPF receptors expressed in CHO-K1 cells in situ.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CHO Cells
  • Cluster Analysis
  • Cricetinae
  • Cricetulus
  • ErbB Receptors / chemistry*
  • ErbB Receptors / metabolism
  • Green Fluorescent Proteins / chemistry
  • Green Fluorescent Proteins / metabolism
  • Ligands*
  • Microscopy, Fluorescence


  • Ligands
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins
  • ErbB Receptors