Abstract
Resveratrol (RSV) has a beneficial role in the prevention of diabetes and alleviates some diabetic complications, such as cardiomyopathy. We investigated cyclooxygenase-1 (COX-1), COX-2, nuclear factor κB (NF-κB), matrix metalloproteinase-9 (MMP-9), and sirtuin 1 (SIRT1) mRNA expression levels in heart tissue after RSV treatment in streptozotocin (STZ)-induced diabetic rats. After induction of chronic diabetes with STZ, 10 mg RSV/kg per day was administered to DM and DM+RSV groups for four weeks. At the end of the experiment, all rats were sacrificed and heart tissues were stored at -80°C; mRNA expression levels of COX-1, COX-2, NF-κB, MMP-9, and SIRT1 genes were analyzed with quantitative real-time PCR. We did not find any significant effect of RSV on MMP-9, COX-1, COX-2, or NF-κB mRNA levels among the groups. However, SIRT1 mRNA levels decreased in the DM group compared to controls and increased in the DM+RSV group when compared to the DM group. SIRT1 is activated by RSV treatment in diabetic heart tissue. Activation of SIRT1 by RSV may lead to a new therapeutic approach for diabetic heart tissue. We conclude that RSV treatment can alleviate heart dysfunction by inhibiton of inflammatory gene expression such as SIRT1.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antioxidants / administration & dosage
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Antioxidants / therapeutic use
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Cardiomyopathies / etiology
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Cardiomyopathies / metabolism*
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Cardiomyopathies / physiopathology
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Cardiomyopathies / prevention & control*
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Cyclooxygenase 1 / genetics
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Cyclooxygenase 1 / metabolism
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Cyclooxygenase 2 / genetics
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Cyclooxygenase 2 / metabolism
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Diabetes Mellitus, Experimental / complications
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Diabetes Mellitus, Experimental / metabolism*
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Diabetes Mellitus, Experimental / physiopathology
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Disease Models, Animal
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Gene Expression Regulation / drug effects*
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Heart / drug effects*
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Heart / physiopathology
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Male
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Matrix Metalloproteinase 9 / genetics
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Matrix Metalloproteinase 9 / metabolism
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NF-kappa B / genetics
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NF-kappa B / metabolism
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RNA, Messenger / analysis
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RNA, Messenger / biosynthesis
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Rats
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Rats, Wistar
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Real-Time Polymerase Chain Reaction
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Resveratrol
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Signal Transduction / drug effects
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Signal Transduction / genetics
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Sirtuin 1 / antagonists & inhibitors*
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Sirtuin 1 / genetics
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Sirtuin 1 / metabolism
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Stilbenes / administration & dosage*
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Stilbenes / therapeutic use
Substances
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Antioxidants
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NF-kappa B
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RNA, Messenger
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Stilbenes
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Cyclooxygenase 1
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Cyclooxygenase 2
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Matrix Metalloproteinase 9
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Sirtuin 1
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Resveratrol