C57BL/6 and 129/Sv mice: genetic difference to renal ischemia-reperfusion

J Nephrol. Sep-Oct 2012;25(5):738-43. doi: 10.5301/jn.5000053.

Abstract

Background: C57BL/6 and 129/Sv are the 2 most commonly used strains of mice in renal ischemia-reperfusion injury (IRI) studies, yet there are currently no studies that contrast differences in the degree of renal injury after ischemia-reperfusion.

Methods: To evaluate renal IRI in male C57BL/6 and 129/Sv mice, we performed unilateral clamping of the left renal pedicle for 45 minutes and compared the degree of renal tissue damage and function. To measure function and tissue damage we examined: glomerular filtration rate (GFR; by inulin clearance), renal blood flow (RBF; by p-aminohippurate [PAH] clearance), renal morphology, immunohistochemistry for infiltrating leukocytes, and fibrogenic markers by Sirius red staining.

Results: After unilateral IRI, 129/sv mice had significantly less GFR and RBF disfunction at both day 14 (d14) and d28. 129/sv mice also had significantly less acute tubular necrosis on d1 and fewer infiltrating leukocytes on d28, as well as less collagen deposition on d28 than C57BL/6 mice.

Conclusions: C57BL/6 mice were much more sensitive to damage caused by renal IRI than are 129/Sv mice.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Biomarkers / metabolism
  • Biopsy
  • Collagen Type I / metabolism
  • Collagen Type III / metabolism
  • Disease Models, Animal
  • Genetic Predisposition to Disease
  • Glomerular Filtration Rate
  • Immunohistochemistry
  • Kidney / blood supply*
  • Kidney / metabolism
  • Kidney / pathology
  • Kidney / physiopathology
  • Lymphocytes / pathology
  • Macrophages / pathology
  • Male
  • Mice
  • Mice, 129 Strain
  • Mice, Inbred C57BL
  • Monocytes / pathology
  • Phenotype
  • Renal Circulation
  • Reperfusion Injury / genetics*
  • Reperfusion Injury / metabolism
  • Reperfusion Injury / pathology
  • Reperfusion Injury / physiopathology
  • Severity of Illness Index
  • Species Specificity
  • Staining and Labeling
  • Time Factors

Substances

  • Biomarkers
  • Collagen Type I
  • Collagen Type III