Association between the rs2910164 polymorphism in pre-mir-146a and oral carcinoma progression

Oral Oncol. 2012 May;48(5):404-8. doi: 10.1016/j.oraloncology.2011.11.019. Epub 2011 Dec 17.

Abstract

MicroRNAs are short non-coding RNAs that regulate gene expression by RNA interference. Oral squamous cell carcinoma (OSCC) is a prevalent malignancy worldwide. miR-146a has been reported to regulate Toll-like receptors and cytokine signaling, which are both crucial for inflammation and oncogenesis. This study identifies that areca nut extract, TNFα and TGFβ up-regulates miR-146a in OSCC cells. The increased expression of miR-146a enhanced the oncogenicity of OSCC cells. In addition, a G to C polymorphism (rs2910164), which is located in the pre-miR-146a and has been associated with functional alterations in miR-146a, was significantly more prevalent among OSCC patients having more advanced nodal involvement. Our analysis also suggested a higher miR-146a expression in OSCC tissues of patients carrying C polymorphism. The present study concluded a higher prevalence of the pre-mir-146a C-variant was associated with OSCC progression in patients with this disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Areca / chemistry
  • Carcinoma, Squamous Cell / genetics*
  • Carcinoma, Squamous Cell / metabolism
  • Case-Control Studies
  • Humans
  • Keratinocytes / drug effects
  • Keratinocytes / metabolism
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Mouth Neoplasms / genetics*
  • Mouth Neoplasms / metabolism
  • NF-kappa B / metabolism
  • NF-kappa B / pharmacology
  • Nuts / chemistry
  • Plant Preparations / pharmacology
  • Polymorphism, Single Nucleotide*
  • Transforming Growth Factor beta / pharmacology
  • Tumor Necrosis Factor-alpha / pharmacology

Substances

  • MIRN146 microRNA, human
  • MicroRNAs
  • NF-kappa B
  • Plant Preparations
  • Transforming Growth Factor beta
  • Tumor Necrosis Factor-alpha