Specific and reversible photochemical labeling of plasmid DNA using photoresponsive oligonucleotides containing 3-cyanovinylcarbazole

Mol Biosyst. 2012 Feb;8(2):491-4. doi: 10.1039/c2mb05422a. Epub 2011 Dec 19.

Abstract

To develop a covalent and specific labeling method for single- and double-stranded plasmid DNA, photoresponsive oligonucleotide containing 3-cyanovinylcarbazole nucleoside was adopted. Single- and double-stranded plasmid DNA was successfully labeled/de-labeled with Cy3 and/or biotin by photoirradiation.

MeSH terms

  • Acrylonitrile / analogs & derivatives
  • Acrylonitrile / chemistry
  • Carbazoles / chemistry
  • DNA / chemistry*
  • Fluorescent Dyes / chemistry
  • Oligonucleotides / chemistry*
  • Photochemical Processes
  • Plasmids / genetics*
  • Staining and Labeling / methods*

Substances

  • Carbazoles
  • Fluorescent Dyes
  • Oligonucleotides
  • DNA
  • Acrylonitrile