Expression of RECK in endothelial cells of glioma: comparison with CD34 and VEGF expressions

J Neurooncol. 2012 May;107(3):559-64. doi: 10.1007/s11060-011-0778-z. Epub 2011 Dec 20.


Angiogenesis is thought to be involved in the progression of glioma grades, and reversion-inducing cysteine-rich protein with Kazal motifs (RECK) has been said to be involved in maturation of vessels. In this study, we aimed to determine whether high micro-vessel density (MVD) expressed by RECK in glioma tissue is correlated with grades of glioma. We also compared RECK expression with that of the formerly known vessel marker, CD34, and vascular endothelial growth factor (VEGF). RECK, CD34, and VEGF immuno-reactivities of 72 glioma tissues were studied. RECK was seen in microvessels of glioma tissues. CD34 showed a similar pattern to RECK, whereas VEGF showed positive staining in cytoplasm of tumor cells and endothelial cells. Average MVD with RECK was 107.6 microvessels (range 7-290). RECK was positively correlated with grades of glioma. RECK and CD34 also showed a strong correlation (P = 0.001). Higher frequency of VEGF staining was also correlated with higher grade of glioma. This is the first study describing expression of RECK in glioma, and its angiogenesis-related nature may provide a potential therapeutic target for glioma treatment in the future.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD34 / biosynthesis*
  • Brain Neoplasms / metabolism*
  • Brain Neoplasms / pathology
  • Endothelial Cells / metabolism
  • Endothelial Cells / pathology
  • GPI-Linked Proteins / biosynthesis*
  • Glioma / metabolism*
  • Glioma / pathology
  • Humans
  • Immunohistochemistry
  • Neoplasm Grading
  • Neovascularization, Pathologic / metabolism
  • Neovascularization, Pathologic / pathology
  • Vascular Endothelial Growth Factor A / biosynthesis*


  • Antigens, CD34
  • GPI-Linked Proteins
  • RECK protein, human
  • Vascular Endothelial Growth Factor A