Isolation and characterization of naïve follicular dendritic cells

Mol Immunol. 2012 Mar;50(3):172-6. doi: 10.1016/j.molimm.2011.11.010. Epub 2011 Dec 19.

Abstract

Follicular dendritic cells (FDC) are specialized antigen-presenting cells to cognate B cells in the follicle of the lymphoid tissues. FDC also support survival and proliferation of the B cells, leading to the germinal center formation. FDC therefore play a central role in humoral immune responses. However, molecular and functional characteristics of FDC are largely unknown, because it is difficult to isolate and analyze FDC due to a very small number of FDC in the lymphoid tissues and the fragility by mechanical and chemical stresses in vitro. In this report, we established a novel method for FDC isolation from the spleen of naïve mice by flow cytometry and analyzed the phenotypical and functional characteristics. The isolated FDC, which accounted for ~0.2% of the spleen cells of naïve mice, were CD45(-), FDC-M2(+), and ICAM-1(+), and supported the survival and LPS-induced proliferation of B cells. We also showed that a neutralizing antibody against B cell activating factor TNF family (BAFF) suppressed FDC-dependent B cell proliferation in the presence of LPS, but not survival, demonstrating the evidence that FDC-derived BAFF is involved in B cell proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Cell Activating Factor / immunology
  • B-Lymphocytes / immunology
  • Cell Culture Techniques / methods*
  • Cell Proliferation
  • Cell Separation / methods*
  • Dendritic Cells, Follicular / cytology*
  • Mice
  • Mice, Inbred C57BL

Substances

  • B-Cell Activating Factor
  • Tnfsf13b protein, mouse