Chemical Compositions and Anti-Influenza Activities of Essential Oils From Mosla Dianthera

J Ethnopharmacol. 2012 Jan 31;139(2):668-71. doi: 10.1016/j.jep.2011.11.056. Epub 2011 Dec 16.

Abstract

Ethnopharmacological relevance: Mosla dianthera as an aromatic herb is used in folk medicine for the treatment of cough, colds, fever, bronchitis, nasal congestion and headache.

Aim of the study: To characterize chemical compositions and to evaluate the anti-influenza effects of essential oils of M. dianthera (MDEO) in influenza virus A (IVA) infected mice.

Materials and methods: MDEO was obtained by hydrodistillation and analysed by gas chromatography-mass spectrometry (GC-MS). ICR mice were treated with MDEO for 5 consecutive days at doses of 90-360 mg/kg after post-infected. Levels of Serum IL-4 and IFN-γ were assayed by ELISA. Levels of MOD, SOD, TAOC and GSH-Px in lung tissue were determined by colorimetric method.

Results: GC-MS analysis revealed the presence of 29 components that account for 97.74% of phenolic sesquiterpenes and aromatic compounds. The major compounds were elemicin (16.51%), thymol (14.77%), β-caryophyllene (14.49%), iso-elemicin (9.22%), asarone (6.09%) and α-caryophyllene (5.26%). It had significant effects on decreasing lung viral titers, inhibiting pneumonia, reducing levels of serum IFN-γ and IL-4, and enhancing antioxidant activity in the lung tissue of IVA infected mice.

Conclusions: MPE could exhibit therapeutical effects in IVA infected mice as a suppressor of IVA replication and inflammatory mediators and a promoter of antioxidant potentials. Therefore, MDEO could provide a safe and effective therapeutic candidate for treatment of influenza and its subsequent viral pneumonia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antiviral Agents / chemistry
  • Antiviral Agents / isolation & purification
  • Antiviral Agents / pharmacology*
  • Antiviral Agents / toxicity
  • Colorimetry
  • Disease Models, Animal
  • Enzyme-Linked Immunosorbent Assay
  • Gas Chromatography-Mass Spectrometry
  • Glutathione Peroxidase / metabolism
  • Inflammation Mediators / blood
  • Influenza A Virus, H1N1 Subtype / drug effects*
  • Influenza A Virus, H1N1 Subtype / pathogenicity
  • Interferon-gamma / blood
  • Interleukin-4 / blood
  • Lamiaceae* / chemistry
  • Lung / drug effects*
  • Lung / metabolism
  • Lung / pathology
  • Lung / virology
  • Malondialdehyde / metabolism
  • Mice
  • Mice, Inbred ICR
  • Oils, Volatile / chemistry
  • Oils, Volatile / isolation & purification
  • Oils, Volatile / pharmacology*
  • Oils, Volatile / toxicity
  • Orthomyxoviridae Infections / blood
  • Orthomyxoviridae Infections / drug therapy*
  • Orthomyxoviridae Infections / pathology
  • Orthomyxoviridae Infections / virology
  • Plant Components, Aerial
  • Plant Oils / chemistry
  • Plant Oils / isolation & purification
  • Plant Oils / pharmacology*
  • Plant Oils / toxicity
  • Plants, Medicinal
  • Ribavirin / pharmacology
  • Superoxide Dismutase / metabolism
  • Virus Replication / drug effects

Substances

  • Antiviral Agents
  • Inflammation Mediators
  • Oils, Volatile
  • Plant Oils
  • Interleukin-4
  • Ribavirin
  • Malondialdehyde
  • Interferon-gamma
  • Glutathione Peroxidase
  • Superoxide Dismutase