BAPTI and BAPTISM birthdating of neurons in zebrafish

Cold Spring Harb Protoc. 2012 Jan 1;2012(1):87-92. doi: 10.1101/pdb.prot067520.

Abstract

This protocol describes how the photoconvertible protein Kaede can be used to determine the birthdates of neurons in live zebrafish. The methods used are birthdating analysis by photoconverted fluorescent protein tracing in vivo (BAPTI) and BAPTI combined with subpopulation markers (BAPTISM). Because Kaede can be converted from green to red fluorescence at any developmental time point, it serves as a temporal landmark for cell birth. When it is used in combination with subpopulation markers, the eventual fate of a cell can be correlated with its birthdate. We describe how we used this method to study the development of trigeminal sensory neurons and discuss how the technique can be extended to the study of other organs.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Developmental Biology / methods*
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / metabolism*
  • Neurons / physiology*
  • Staining and Labeling / methods*
  • Trigeminal Nerve / cytology
  • Trigeminal Nerve / growth & development*
  • Zebrafish / growth & development*

Substances

  • Fluorescent Dyes