Linear sweep voltammetric studies on the complex of alizarin red s with aloe polysaccharide and determination of aloe polysaccharide

Carbohydr Res. 2012 Feb 15:349:82-5. doi: 10.1016/j.carres.2011.11.026. Epub 2011 Dec 6.

Abstract

A new electrochemical method for the determination of aloe polysaccharide based on its interaction with alizarin red s was established on a pretreated glassy carbon electrode in pH 3.5 Britton-Robinson buffer solution by linear sweep voltammetry in this work. The decrease of the second order derivative linear sweep voltammetric reductive peak current of alizarin red s is in proportion to aloe polysaccharide concentration due to the formation of a complex between them in the range from 0.032 to 0.448μmolL(-1), and the detection limit is 0.0051μmolL(-1) (3σ). The binding ratio and the binding constant of the complex were calculated as 1:1 and 2.75×10(4), respectively. Different kinds of complex samples of aloe polysaccharide were detected satisfactorily by this method.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aloe / chemistry*
  • Anthraquinones / chemistry*
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Electrochemistry / methods*
  • Electrodes
  • Molecular Sequence Data
  • Polysaccharides / analysis*

Substances

  • Anthraquinones
  • Polysaccharides
  • Alizarin Red S