Characterisation of Chlamydia psittaci isolated from a horse

Vet Microbiol. 1990 Jul;24(1):11-9. doi: 10.1016/0378-1135(90)90046-x.


This paper describes the isolation and characterisation of a strain of Chlamydia psittaci obtained from a nasal swab taken from a horse with serous nasal discharge. Initial isolation was achieved in cycloheximide-treated McCoy cell monolayers. Chlamydial inclusions stained by immunofluorescence either with a rabbit antiserum raised against C. psittaci or with a monoclonal antibody directed against the genus-specific lipopolysaccharide antigen were single and compact. They did not stain with iodine or with a monoclonal antibody reactive against Chlamydia trachomatis. The agent was re-isolated in the yolk sacs of embryonated hens eggs and designated N16. Identification of the agent was confirmed by electron microscopy. Unique plasmid DNA was prepared from a purified suspension of chlamydial elementary bodies (EBs), and analysed by electrophoresis through 1.0% agarose gels stained by ethidium bromide. This strain of C. psittaci grew relatively slowly in cycloheximide-treated McCoy cells, and the yield of elementary bodies during the course of one growth cycle was relatively low.

Publication types

  • Case Reports

MeSH terms

  • Animals
  • Cell Line
  • Centrifugation
  • Chlamydophila psittaci / genetics
  • Chlamydophila psittaci / growth & development
  • Chlamydophila psittaci / isolation & purification*
  • Chlamydophila psittaci / ultrastructure
  • DNA, Bacterial / analysis
  • Fluorescent Antibody Technique
  • Horse Diseases / microbiology*
  • Horses
  • Male
  • Microscopy, Electron
  • Nasal Mucosa / microbiology
  • Plasmids
  • Psittacosis / microbiology
  • Psittacosis / veterinary*
  • Respiratory Tract Infections / microbiology
  • Respiratory Tract Infections / veterinary*


  • DNA, Bacterial