Lifetimes of photosystem I and II proteins in the cyanobacterium Synechocystis sp. PCC 6803

FEBS Lett. 2012 Jan 20;586(2):169-73. doi: 10.1016/j.febslet.2011.12.010. Epub 2011 Dec 17.

Abstract

The half-life times of photosystem I and II proteins were determined using (15)N-labeling and mass spectrometry. The half-life times (30-75h for photosystem I components and <1-11h for the large photosystem II proteins) were similar when proteins were isolated from monomeric vs. oligomeric complexes on Blue-Native gels, suggesting that the two forms of both photosystems can interchange on a timescale of <1h or that only one form of each photosystem exists in thylakoids in vivo. The half-life times of proteins associated with either photosystem generally were unaffected by the absence of Small Cab-like proteins.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cells, Cultured
  • Cyanobacteria / enzymology
  • Cyanobacteria / genetics
  • Cyanobacteria / metabolism
  • Half-Life
  • Multiprotein Complexes / chemistry
  • Multiprotein Complexes / metabolism
  • Photosynthetic Reaction Center Complex Proteins / chemistry
  • Photosynthetic Reaction Center Complex Proteins / metabolism
  • Photosystem I Protein Complex / genetics
  • Photosystem I Protein Complex / metabolism*
  • Photosystem I Protein Complex / physiology
  • Photosystem II Protein Complex / genetics
  • Photosystem II Protein Complex / metabolism*
  • Photosystem II Protein Complex / physiology
  • Proteolysis*
  • Synechocystis / enzymology
  • Synechocystis / genetics
  • Synechocystis / metabolism*
  • Time Factors

Substances

  • Multiprotein Complexes
  • Photosynthetic Reaction Center Complex Proteins
  • Photosystem I Protein Complex
  • Photosystem II Protein Complex