Purification of L-alpha glycerylphosphorylcholine by column chromatography

J Chromatogr A. 2012 Jan 13;1220:108-14. doi: 10.1016/j.chroma.2011.12.003. Epub 2011 Dec 11.

Abstract

Colorless L-alpha glycerylphosphorylcholine (L-α-GPC) was obtained at 99.8% purity, 69.8% recovery, and with a specific rotation of -2.5° via a five-step procedure. L-α-GPC was first produced by phospholipase A(1) hydrolysis of soy lecithin powder. Ca(2+) and Cl(-) were then effectively removed using two successive 001×7 cation and D311 anion exchange resin column chromatography procedures. Silica gel column chromatography and decoloration with active carbon were then applied to remove remaining impurities and colorant. Characterization of the L-α-GPC product was well in agreement with the standard. The resin and silica gel showed remarkable ability for L-α-GPC isolation after 10 uses. Thus, this study presents a simple and cost-effective method for preparing L-α-GPC with high yield and purity, low cost, and environmental friendliness, and encourages future investigation into its adaptation for industrial applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Calcium / chemistry
  • Chlorides / chemistry
  • Chromatography, High Pressure Liquid / methods*
  • Chromatography, Ion Exchange / methods*
  • Equipment Reuse
  • Glycerylphosphorylcholine / isolation & purification*
  • Ion Exchange Resins
  • Lecithins / chemistry
  • Lecithins / metabolism
  • Methanol
  • Phospholipases A1 / metabolism
  • Silica Gel
  • Soybeans

Substances

  • Chlorides
  • Ion Exchange Resins
  • Lecithins
  • Silica Gel
  • Glycerylphosphorylcholine
  • Phospholipases A1
  • Calcium
  • Methanol