Cohnella soli sp. nov. and Cohnella suwonensis sp. nov. Isolated from soil samples in Korea

J Microbiol. 2011 Dec;49(6):1033-8. doi: 10.1007/s12275-011-1071-8. Epub 2011 Dec 28.

Abstract

Two bacterial isolates from soil samples taken in Korea, strains YM2-7(T) and WD2-19(T), were characterized using a polyphasic approach. The cells were strictly aerobic, Gram-positive, motile with peritrichous flagella, and rod-shaped. Both strains formed ellipsoidal bulging positioned subterminal spores. Phylogenetic analysis of their 16S rRNA gene sequences revealed a clear affiliation with the Firmicutes. The 16S rRNA gene sequence similarity between YM2-7(T) and WD2-19(T) was 96.5%. Strains YM2-7(T) and WD2-19(T) showed 16S rRNA gene sequence similarities of 93.0-96.5% to type strains of recognized Cohnella species. The G+C contents of the DNA of strains YM2-7(T) and WD2-19(T) were 52.2 and 55.6 mol%, respectively. The major fatty acids of strains YM2-7(T) and WD2-19(T) were anteiso-C15:0 (44.4%), C16:0 (19.2%), and iso-C16:0 (16.8%) and anteiso-C15:0 (46.5%), iso-C16:0 (21.8%), and C16:0 (11.2%), respectively. Both strains contained menaquinone with seven isoprene units (MK-7) as the predominant quinone. Both strains had diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and lysophosphatidylglycerol as the major polar lipids. Comparative analysis of phenotypic and phylogenetic traits indicated that strains YM2-7(T) and WD2-19(T) represented two novel species of the genus Cohnella. The names Cohnella soli sp. nov. (type strain YM2-7(T) =KACC 13346(T) =NBRC 106486(T)), and Cohnella suwonensis sp. nov. (type strain WD2-19T =KACC 13347(T) =NBRC 106485(T)) are proposed for these organisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillales / classification*
  • Bacillales / genetics
  • Bacillales / isolation & purification*
  • Base Composition
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / genetics
  • Fatty Acids / metabolism
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Republic of Korea
  • Soil Microbiology*

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/GU181266
  • GENBANK/GU181267