An increasing number of university students are earning their degrees online. Thus far, the determinants of health among online university students have not been studied. The purpose of this cross-sectional internet survey was to test the association between behavioral risk factors and self-rated health in a diverse sample of online university students. Cigarette smoking was associated with lower odds of good self-rated health (OR = 0.27, P < 0.01). Compared to having zero days of mental distress, 14 or more days of mental distress was associated with lower odds of good self-rated health (OR = 0.08, P < 0.05). White students had lower odds of reporting good self-rated health compared to African-American students (OR = 0.35, P < 0.05). Health education, screening and health promotion programs should be developed for online students.