Inter-laboratory assessment of a harmonized zebrafish developmental toxicology assay - progress report on phase I

Reprod Toxicol. 2012 Apr;33(2):155-64. doi: 10.1016/j.reprotox.2011.12.004. Epub 2011 Dec 21.


This report provides a progress update of a consortium effort to develop a harmonized zebrafish developmental toxicity assay. Twenty non-proprietary compounds (10 animal teratogens and 10 animal non-teratogens) were evaluated blinded in 4 laboratories. Zebrafish embryos from pond-derived and cultivated strain wild types were exposed to the test compounds for 5 days and subsequently evaluated for lethality and morphological changes. Each of the testing laboratories achieved similar overall concordance to the animal data (60-70%). Subsequent optimization procedures to improve the overall concordance focused on compound formulation and test concentration adjustments, chorion permeation and number of replicates. These optimized procedures were integrated into a revised protocol and all compounds were retested in one lab using embryos from pond-derived zebrafish and achieved 85% total concordance. To further assess assay performance, a study of additional compounds is currently in progress at two laboratories using embryos from pond-derived and cultivated-strain wild type zebrafish.

Publication types

  • Evaluation Study

MeSH terms

  • Abnormalities, Drug-Induced
  • Animals
  • Drug Evaluation, Preclinical / methods
  • Drug Evaluation, Preclinical / standards*
  • Embryo, Nonmammalian / drug effects*
  • Models, Animal
  • Reproducibility of Results
  • Research Report
  • Teratogens / toxicity*
  • Toxicity Tests / methods
  • Toxicity Tests / standards*
  • Zebrafish*


  • Teratogens