Creation of an artificial metalloprotein with a Hoveyda-Grubbs catalyst moiety through the intrinsic inhibition mechanism of α-chymotrypsin

Takashi Matsuo; Chie Imai; Takefumi Yoshida; Takashi Saito; Takashi Hayashi; Shun Hirota

doi:10.1039/c2cc16898g

Creation of an artificial metalloprotein with a Hoveyda-Grubbs catalyst moiety through the intrinsic inhibition mechanism of α-chymotrypsin

Chem Commun (Camb). 2012 Feb 4;48(11):1662-4. doi: 10.1039/c2cc16898g. Epub 2012 Jan 3.

Authors

Takashi Matsuo¹, Chie Imai, Takefumi Yoshida, Takashi Saito, Takashi Hayashi, Shun Hirota

Affiliation

¹ Graduate School of Materials Science, Nara Institute of Science and Technology (NAIST), Ikoma, Nara 630-0192, Japan. tmatsuo@ms.naist.jp

PMID: 22212522
DOI: 10.1039/c2cc16898g

Abstract

An L-phenylalanyl chloromethylketone-based inhibitor equipped with a Hoveyda-Grubbs catalyst moiety was regioselectively incorporated into the cleft of α-chymotrypsin through the intrinsic inhibition mechanism of the protein to construct an artificial organometallic protein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Catalysis
Chymotrypsin / chemistry*
Circular Dichroism
Metalloproteins / chemical synthesis*
Protein Conformation
Ruthenium / chemistry
Spectrometry, Mass, Electrospray Ionization
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tosylphenylalanyl Chloromethyl Ketone / chemistry

Substances

Metalloproteins
Tosylphenylalanyl Chloromethyl Ketone
Ruthenium
Chymotrypsin
alpha-chymotrypsin