Extraction method for preparing pyridylamino sugar derivatives and application to porcine gastric mucus glycoprotein analysis

Anal Biochem. 1990 Jul;188(1):200-2. doi: 10.1016/0003-2697(90)90552-k.


For the sensitive detection of free sugars and oligosaccharides, the use of their pyridylamino derivatives has now found general acceptance. To remove excess 2-aminopyridine from this derivative in a reaction mixture, gel filtration and ion-exchange chromatography were conducted. It was found in the present study that contaminated 2-aminopyridine could be selectively removed from the reaction mixture by adjusting the pH with saturated sodium bicarbonate at above 8.5 followed by extraction with benzene. By using this method, fewer purification steps and less time are required, with minimum loss of pyridylamino sugar derivatives.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Sugars / chemical synthesis
  • Amino Sugars / isolation & purification*
  • Aminopyridines / isolation & purification*
  • Animals
  • Carbohydrates / analysis
  • Chromatography, High Pressure Liquid
  • Chromatography, Ion Exchange
  • Glycoproteins / analysis*
  • Indicators and Reagents
  • Mucus*
  • Swine


  • Amino Sugars
  • Aminopyridines
  • Carbohydrates
  • Glycoproteins
  • Indicators and Reagents
  • alpha-aminopyridine