Bone regeneration in a rabbit critical-sized calvarial model using tyrosine-derived polycarbonate scaffolds

Tissue Eng Part A. 2012 Jun;18(11-12):1132-9. doi: 10.1089/ten.TEA.2011.0582. Epub 2012 Feb 8.


Porous three-dimensional tyrosine-derived polycarbonate (TyrPC) scaffolds with a bimodal pore distribution were fabricated to mimic bone architecture using a combination of salt-leaching and phase separation techniques. TyrPC scaffolds degraded in register with bone regeneration during the 6-week study period and compressive moduli of the scaffolds were maintained >0.5 MPa at 6 weeks of incubation in PBS at 37 °C. The TyrPC scaffolds either unsupplemented or supplemented with recombinant human bone morphogenetic protein-2 (rhBMP-2) were implanted in a rabbit calvarial critical-sized defect (CSD) model and the TyrPC scaffolds treated with rhBMP-2 or TyrPC coated with calcium phosphate scaffold alone promoted bone regeneration in a rabbit calvarial CSD at 6 weeks postimplantation. A synthetic TyrPC polymeric scaffold either without a biological supplement or with a minimal dose of rhBMP-2 induced bone regeneration comparable to a commercially available bone graft substitute in a nonrodent CSD animal model.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Bone Morphogenetic Protein 2 / pharmacology
  • Bone Regeneration / drug effects*
  • Cattle
  • Disease Models, Animal
  • Elastic Modulus / drug effects
  • Humans
  • Implants, Experimental
  • Male
  • Polycarboxylate Cement / pharmacology*
  • Rabbits
  • Recombinant Proteins / pharmacology
  • Skull / diagnostic imaging
  • Skull / drug effects*
  • Skull / pathology*
  • Tissue Scaffolds / chemistry*
  • Transforming Growth Factor beta / pharmacology
  • Tyrosine / pharmacology*
  • X-Ray Microtomography


  • Bone Morphogenetic Protein 2
  • Polycarboxylate Cement
  • Recombinant Proteins
  • Transforming Growth Factor beta
  • recombinant human bone morphogenetic protein-2
  • polycarbonate
  • Tyrosine