Monitoring of cellular senescence by DNA-methylation at specific CpG sites

Aging Cell. 2012 Apr;11(2):366-9. doi: 10.1111/j.1474-9726.2011.00784.x. Epub 2012 Jan 19.


Replicative senescence has fundamental implications on cell morphology, proliferation, and differentiation potential. Here, we describe a simple method to track long-term culture based on continuous DNA-methylation changes at six specific CpG sites. This epigenetic senescence signature can be used as biomarker for various cell types to predict the state of cellular senescence with regard to the number of passages, population doublings, or days of in vitro culture.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Cellular Senescence*
  • CpG Islands*
  • DNA Methylation*
  • Epigenesis, Genetic
  • Humans