Aptamer-DNAzyme hairpins for biosensing of Ochratoxin A

Cheng Yang; Vasilica Lates; Beatriz Prieto-Simón; Jean-Louis Marty; Xiurong Yang

doi:10.1016/j.bios.2011.12.011

Aptamer-DNAzyme hairpins for biosensing of Ochratoxin A

Biosens Bioelectron. 2012 Feb 15;32(1):208-12. doi: 10.1016/j.bios.2011.12.011. Epub 2011 Dec 13.

Authors

Cheng Yang¹, Vasilica Lates, Beatriz Prieto-Simón, Jean-Louis Marty, Xiurong Yang

Affiliation

¹ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.

PMID: 22221796
DOI: 10.1016/j.bios.2011.12.011

Abstract

We report an aptasensor for biosensing of Ochratoxin A (OTA) using aptamer-DNAzyme hairpin as biorecognition element. The structure of this engineered nucleic acid includes the horseradish peroxidase (HRP)-mimicking DNAzyme and the OTA specific aptamer sequences. A blocking tail captures a part of these sequences in the stem region of the hairpin. In the presence of OTA, the hairpin is opened due to the formation of the aptamer-analyte complex. As a result, self-assembly of the active HRP-mimicking DNAzyme occurs. The activity of this DNAzyme is linearly correlated with OTA concentration up to 10 nM, showing a limit of detection of 2.5 nM.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide / chemistry*
Base Sequence
Biosensing Techniques / methods*
DNA, Catalytic / chemistry*
G-Quadruplexes
Limit of Detection
Molecular Sequence Data
Mycotoxins / analysis*
Ochratoxins / analysis*
Wine / analysis*

Substances

Aptamers, Nucleotide
DNA, Catalytic
Mycotoxins
Ochratoxins
ochratoxin A