Stimulation of Mn-superoxide dismutase expression by tumor necrosis factor-alpha: quantitative determination of Mn-SOD protein levels in TNF-resistant and sensitive cells by ELISA

Biochem Biophys Res Commun. 1990 Sep 28;171(3):1378-86. doi: 10.1016/0006-291x(90)90838-e.

Abstract

Marked increase in protein levels of Mn-superoxide dismutase (Mn-SOD) was found in TNF-resistant cell lines after treatment with Tumor Necrosis Factor (TNF). No such increase was observed in Cu,Zn-superoxide dismutase (Cu, Zn-SOD) protein in either TNF-resistant or sensitive cells. These results support the data that the Mn-SOD is one of the rescue proteins required for resistance to TNF cytotoxicity in these cell lines (Wong et al., Cell 58, 923-931, 1990). Mn-SOD was also responsive to TNF stimulation in KURAMOCHI, a human ovarian adenocarcinoma cell line. This may explain our previous result that Mn-SOD protein is highly expressed in epithelial ovarian cancer (Ishikawa et al. Cancer Res. 50, 2538-2542, 1990).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma
  • Cell Line
  • Cell Survival / drug effects
  • Drug Resistance
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Kinetics
  • Ovarian Neoplasms
  • Recombinant Proteins / pharmacology
  • Superoxide Dismutase / biosynthesis
  • Superoxide Dismutase / metabolism*
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha / pharmacology*

Substances

  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • Superoxide Dismutase