Laser microsurgery in Caenorhabditis elegans

Methods Cell Biol. 2012;107:177-206. doi: 10.1016/B978-0-12-394620-1.00006-0.

Abstract

Laser killing of cell nuclei has long been a powerful means of examining the roles of individual cells in C. elegans. Advances in genetics, laser technology, and imaging have further expanded the capabilities and usefulness of laser surgery. Here, we review the implementation and application of currently used methods for target edoptical disruption in C. elegans.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Animals
  • Axotomy
  • Caenorhabditis elegans / cytology
  • Caenorhabditis elegans / physiology*
  • Caenorhabditis elegans / radiation effects
  • Cell Lineage
  • Cell Nucleus / radiation effects
  • Cell Nucleus / ultrastructure
  • Fluorescent Dyes
  • Green Fluorescent Proteins
  • Larva / cytology
  • Larva / physiology*
  • Larva / radiation effects
  • Laser Therapy / methods*
  • Lasers
  • Low-Level Light Therapy
  • Microfluidics
  • Microsurgery / methods*
  • Neurons / physiology*
  • Neurons / radiation effects
  • Photosensitizing Agents

Substances

  • Fluorescent Dyes
  • Photosensitizing Agents
  • killer red protein, Anthomedusae
  • Green Fluorescent Proteins