Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton

Nat Methods. 2012 Jan 8;9(2):185-8. doi: 10.1038/nmeth.1841.


By combining astigmatism imaging with a dual-objective scheme, we improved the image resolution of stochastic optical reconstruction microscopy (STORM) and obtained <10-nm lateral resolution and <20-nm axial resolution when imaging biological specimens. Using this approach, we resolved individual actin filaments in cells and revealed three-dimensional ultrastructure of the actin cytoskeleton. We observed two vertically separated layers of actin networks with distinct structural organizations in sheet-like cell protrusions.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / chemistry*
  • Cell Line
  • Cytoskeleton / chemistry*


  • Actins