Pyrrolysine analogs as substrates for bacterial pyrrolysyl-tRNA synthetase in vitro and in vivo

Hidekazu Katayama; Kayo Nozawa; Osamu Nureki; Yoshiaki Nakahara; Hironobu Hojo

doi:10.1271/bbb.110653

Pyrrolysine analogs as substrates for bacterial pyrrolysyl-tRNA synthetase in vitro and in vivo

Biosci Biotechnol Biochem. 2012;76(1):205-8. doi: 10.1271/bbb.110653. Epub 2012 Jan 7.

Authors

Hidekazu Katayama¹, Kayo Nozawa, Osamu Nureki, Yoshiaki Nakahara, Hironobu Hojo

Affiliation

¹ Department of Applied Biochemistry, School of Engineering, Tokai University, Hiratsuka, Kanagawa, Japan. katay@tokai-u.jp

PMID: 22232266
DOI: 10.1271/bbb.110653

Abstract

Pyrrolysine-tRNA(Pyl) complex is produced by pyrrolysyl-tRNA synthetase (PylRS). In this study, we investigated the substrate specificity of Desulfitobacterium hafnience PylRS. PylRS incorporated various L-lysine derivatives into tRNA(Pyl) in vitro. In addition, the PylRS/tRNA(Pyl) pair introduced these lysine derivatives into the recombinant protein by the Escherichia coli expression system, indicating that this PylRS/tRNA(Pyl) pair can be used in protein engineering technology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acyl-tRNA Synthetases / genetics
Amino Acyl-tRNA Synthetases / metabolism*
Desulfitobacterium / enzymology*
Escherichia coli / genetics
Lysine / analogs & derivatives*
Lysine / chemistry
Lysine / metabolism
Protein Binding
Substrate Specificity

Substances

Amino Acyl-tRNA Synthetases
pyrrolysine
Lysine