[Synthesis of 3-hydroxybutyrate-CO-4-hydroxybutyrate copolymers by hydrogen-oxidizing bacteria]

Prikl Biokhim Mikrobiol. 2011 Sep-Oct;47(5):544-50.
[Article in Russian]


Synthesis of 3- and 4-hydroxybutyrate copolymer (3HB-CO-4HB), the most promising member of the biodegradable polyhydroxyalcanoate (PHA) family, has been studied. Cultivation conditions of naturally occurring strains of hydrogen-oxidizing bacteria Ralstonia eutropha B5786 and Cupriavidus eutrophus B10646 have been optimized to ensure efficient synthesis of the 3HB-CO-4HB copolymer. A set of highly pure samples of the 3HB-CO-4HB copolymer with 4HB content varying from 8.7 to 24.3 mol % has been obtained. Incorporation of 4-HB into the copolymer was shown to cause a more pronounced decrease in polymer crystallinity than the incorporation of 3-hydroxyvalerate or 3-hydroxyhexanoate; samples with a degree of crystallinity below 30% have been obtained. The weight average molecular mass of the 3HB-CO-4HB copolymers was shown to be independent on the monomer ratio and to vary broadly (from 540 to 1110 kDa).

Publication types

  • English Abstract

MeSH terms

  • Cell Culture Techniques
  • Crystallography
  • Culture Media / chemistry
  • Cupriavidus necator / genetics
  • Cupriavidus necator / growth & development*
  • Cupriavidus necator / metabolism*
  • Hydrogen / metabolism*
  • Hydroxybutyrates / chemistry
  • Hydroxybutyrates / metabolism*
  • Oxidation-Reduction
  • Polyesters / chemistry
  • Polyesters / metabolism*


  • Culture Media
  • Hydroxybutyrates
  • Polyesters
  • poly(3-hydroxybutyrate-co-4-hydroxybutyrate)
  • Hydrogen