Juniperus oblonga Bieb is widely spread in the Caucasus Mountains, particularly in its eastern and southern regions. Diuretic effect of juniper berries is determined by the presence of volatile oils and polyphenol complex, particularly flavonoids. Flavonoids were extracted from raw material with 70% ethanol and then with ethyl acetate. Column chromatography of ethyl acetate fraction on polyamide yielded 5 compounds, which were identified on the basis of physicochemical constants of parent compounds and products of acid hydrolysis and alkaline degradation of aglycones and on the basis of UV-spectroscopy as apigenin, isoquercitrin, apigenin-7-glucoside, quercetin-3-rutinoside, and scutellarin-7-glucoside. Quantitative composition of flavonoid in equivalent to rutin concentration in Juniperus oblonga Bieb was 0.910±0.007% (UV-spectrophotometry data).