Protective Effects of β-glucan Against Oxidative Injury Induced by 2.45-GHz Electromagnetic Radiation in the Skin Tissue of Rats

Arch Dermatol Res. 2012 Sep;304(7):521-7. doi: 10.1007/s00403-012-1205-9. Epub 2012 Jan 12.

Abstract

In recent times, there is widespread use of 2.45-GHz irradiation-emitting devices in industrial, medical, military and domestic application. The aim of the present study was to investigate the effect of 2.45-GHz electromagnetic radiation (EMR) on the oxidant and antioxidant status of skin and to examine the possible protective effects of β-glucans against the oxidative injury. Thirty-two male Wistar albino rats were randomly divided into four equal groups: control; sham exposed; EMR; and EMR + β-glucan. A 2.45-GHz EMR emitted device from the experimental exposure was applied to the EMR group and EMR + β-glucan group for 60 min daily, respectively, for 4 weeks. β-glucan was administered via gavage at a dose of 50 mg/kg/day before each exposure to radiation in the treatment group. The activities of antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), as well as the concentration of malondialdehyde (MDA) were measured in tissue homogenates of the skin. Exposure to 2.45-GHz EMR caused a significant increase in MDA levels and CAT activity, while the activities of SOD and GSH-Px decreased in skin tissues. Systemic β-glucan significantly reversed the elevation of MDA levels and the reduction of SOD activities. β-glucan treatment also slightly enhanced the activity of CAT and prevented the depletion of GSH-Px activity caused by EMR, but not statistically significantly. The present study demonstrated the role of oxidative mechanisms in EMR-induced skin tissue damages and that β-glucan could ameliorate oxidative skin injury via its antioxidant properties.

MeSH terms

  • Animals
  • Antioxidants / administration & dosage*
  • Catalase / metabolism
  • Electromagnetic Radiation*
  • Enzyme Activation / drug effects
  • Glutathione Peroxidase / metabolism
  • Male
  • Malondialdehyde / metabolism
  • Oxidative Stress / drug effects
  • Radiodermatitis / metabolism
  • Radiodermatitis / prevention & control*
  • Rats
  • Rats, Wistar
  • Skin / drug effects*
  • Skin / injuries
  • Skin / metabolism
  • Skin / radiation effects
  • Superoxide Dismutase / metabolism
  • beta-Glucans / administration & dosage*

Substances

  • Antioxidants
  • beta-Glucans
  • Malondialdehyde
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase