Time course of prion seeding activity in cerebrospinal fluid of scrapie-infected hamsters after intratongue and intracerebral inoculations

J Clin Microbiol. 2012 Apr;50(4):1464-6. doi: 10.1128/JCM.06099-11. Epub 2012 Jan 11.


To assess prospects for early diagnosis of prion disease based on prion seeding activity in cerebrospinal fluid (CSF), we measured the activity over time in scrapie-infected hamsters by real-time quaking-induced conversion (RT-QuIC). After intracerebral inoculation, activity appeared in CSF within 1 day and plateaued weeks before the onset of clinical signs. However, after intratongue inoculation, activity first appeared in CSF with the onset of clinical signs, well after higher-level accumulation of seeding activity in brain.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Amyloid / cerebrospinal fluid*
  • Amyloid / chemistry
  • Animals
  • Brain / pathology
  • Cricetinae
  • Kinetics
  • Limit of Detection
  • Prions / cerebrospinal fluid*
  • Prions / chemistry
  • Scrapie / cerebrospinal fluid*
  • Scrapie / diagnosis
  • Tongue / pathology


  • Amyloid
  • Prions