Identification and characterization of an LCAT-like Arabidopsis thaliana gene encoding a novel phospholipase A

FEBS Lett. 2012 Feb 17;586(4):373-7. doi: 10.1016/j.febslet.2011.12.034. Epub 2012 Jan 10.

Abstract

A previously uncharacterized Arabidopsis lecithin:cholesterol acyltransferase (LCAT) family gene (At4g19860) was functionally expressed in yeast, where it was demonstrated to encode a novel cytosolic and calcium-independent phospholipase A with preferences for the sn-2 position. This enzyme shows optimal activity at pH 5.0, exhibits a headgroup specificity for phosphatidylcholine>phosphatidic acid>phosphatidylethanolamine>phosphatidylglycerol>phosphatidylserine and has an acyl chain specificity for oleoyl>linoleoyl>ricinoleoyl. The expression of AtLCAT-PLA inhibited yeast cell growth and fatty acid accumulation. AtLCAT-PLA transcript in Arabidopsis was detected at high levels in roots and siliques.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / enzymology*
  • Arabidopsis / genetics*
  • Arabidopsis Proteins / genetics*
  • Arabidopsis Proteins / metabolism
  • Base Sequence
  • DNA, Plant / genetics
  • Genes, Plant*
  • Hydrogen-Ion Concentration
  • Molecular Sequence Data
  • Phosphatidylcholine-Sterol O-Acyltransferase / genetics*
  • Phosphatidylcholine-Sterol O-Acyltransferase / metabolism
  • Phosphatidylcholines / metabolism
  • Phospholipases A / genetics*
  • Phospholipases A / metabolism
  • Phylogeny
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Saccharomyces cerevisiae / genetics
  • Substrate Specificity

Substances

  • Arabidopsis Proteins
  • DNA, Plant
  • Phosphatidylcholines
  • Recombinant Proteins
  • Phosphatidylcholine-Sterol O-Acyltransferase
  • Phospholipases A