Glucose ingestion acutely lowers pulsatile LH and basal testosterone secretion in men

Am J Physiol Endocrinol Metab. 2012 Mar 15;302(6):E724-30. doi: 10.1152/ajpendo.00520.2011. Epub 2012 Jan 17.


Chronic hyperglycemia inhibits the male gonadal axis. The present analyses test the hypothesis that acute glucose ingestion also suppresses LH and testosterone (T) secretion and blunts the LH-T dose-response function. The design comprised a prospectively randomized crossover comparison of LH and T secretion after glucose vs. water ingestion in a Clinical Translational Research Center. The participants were healthy men (n = 57) aged 19-78 yr with body mass index (BMI) of 20-39 kg/m(2). The main outcome measurements were deconvolution and LH-T dose-response analyses of 10-min data. LH-T responses were regressed on glucose, insulin, leptin, adiponectin, age, BMI, and CT-estimated abdominal visceral fat. During the first 120 min after glucose ingestion, for each unit decrease in LH concentrations, T concentrations decreased by 86 (27-144) ng/dl (r = 0.853, P < 0.001). Based upon deconvolution analysis, glucose compared with water ingestion reduced 1) basal (nonpulsatile; P < 0.001) and total (P < 0.001) T secretion without affecting pulsatile T output and 2) pulsatile (P = 0.043) but not basal LH secretion. By multivariate analysis, pulsatile LH secretion positively predicted basal T secretion after glucose ingestion (r = 0.374, P = 0.0042). In addition, the glucose-induced fall in pulsatile LH secretion was exacerbated by higher fasting insulin concentrations (P = 0.054) and attenuated by higher adiponectin levels (P = 0.0037). There were no detectable changes in the analytically estimated LH-T dose-response curves (P > 0.30). In conclusion, glucose ingestion suppresses pulsatile LH and basal T secretion acutely in healthy men. Suppression is influenced by age, glucose, adiponectin, and insulin concentrations.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Abdominal Fat / physiology
  • Adiponectin / blood
  • Adult
  • Aged
  • Aging / metabolism
  • Analysis of Variance
  • Blood Glucose / metabolism
  • Body Mass Index
  • Cross-Over Studies
  • Depression, Chemical
  • Dose-Response Relationship, Drug
  • Glucose / pharmacology*
  • Glucose Tolerance Test
  • Humans
  • Insulin Resistance
  • Linear Models
  • Luteinizing Hormone / metabolism*
  • Male
  • Middle Aged
  • Prospective Studies
  • Testosterone / metabolism*
  • Young Adult


  • Adiponectin
  • Blood Glucose
  • Testosterone
  • Luteinizing Hormone
  • Glucose