Soft fruit traceability in food matrices using real-time PCR

Nutrients. 2009 Feb;1(2):316-28. doi: 10.3390/nu1020316. Epub 2009 Dec 23.


Food product authentication provides a means of monitoring and identifying products for consumer protection and regulatory compliance. There is a scarcity of analytical methods for confirming the identity of fruit pulp in products containing Soft Fruit. In the present work we have developed a very sensible qualitative and quantitative method to determine the presence of berry DNAs in different food matrices. To our knowledge, this is the first study that shows the applicability, to Soft Fruit traceability, of melting curve analysis and multiplexed fluorescent probes, in a Real-Time PCR platform. This methodology aims to protect the consumer from label misrepresentation.

Keywords: blueberry; currant; orange; pineapple; raspberry; real-time PCR; soft fruit; strawberry; traceability.

MeSH terms

  • Beverages / analysis
  • Commerce
  • DNA, Plant / genetics*
  • Food Analysis / methods*
  • Fruit / genetics*
  • Plants / genetics*
  • Public Health
  • Real-Time Polymerase Chain Reaction / methods*


  • DNA, Plant